Origin of the white shark Carcharodon (Lamniformes: Lamnidae) based on recalibration of the Upper Neogene Pisco Formation of Peru

Abstract: The taxonomic origin of the white shark, Carcharodon, is a highly debated subject. New fossil evidence presented in this study suggests that the genus is derived from the broad‐toothed ‘mako’, Carcharodon (Cosmopolitodus) hastalis, and includes the new species C. hubbelli sp. nov. – a taxon that demonstrates a transition between C. hastalis and Carcharodon carcharias. Specimens from the Pisco Formation clearly demonstrate an evolutionary mosaic of characters of both recent C. carcharias and fossil C. hastalis. Characters diagnostic to C. carcharias include the presence tooth serrations and a symmetrical first upper anterior tooth that is the largest in the tooth row, while those indicative of C. hastalis include a mesially slanted third anterior (intermediate) tooth. We also provide a recalibration of critical fossil horizons within the Pisco Formation, Peru using zircon U‐Pb dating and strontium‐ratio isotopic analysis. The recalibration of the absolute dates suggests that Carcharodon hubbelli sp. nov. is Late Miocene (6–8 Ma) in age. This research revises and elucidates lamnid shark evolution based on the calibration of the Neogene Pisco Formation.     

Fruit Production in Mature and Recently Regenerated Forests of the Appalachians

ABSTRACT Fleshy fruit is a key food resource for both game and nongame wildlife, and it may be especially important for migratory birds during fall and for resident birds and mammals during winter. Land managers need to know how land uses affect the quantities and species of fruit produced in different forest types and how fruit production varies seasonally and as young stands mature. During June 1999-April 2004, we quantified fleshy fruit abundance monthly in 31 0.1-ha plots in 2 silvicultural treatments: 1) young 2-age stands with low basal area retention, created by shelterwood-with-reserves regeneration cuts (R; harvested 1998–1999); and 2) uncut mature closed-canopy stands (M) in 2 common southern Appalachian, USA, forest types (upland hardwood and cove hardwood [CH] forests). Over the 5-year study period, total dry pulp biomass production was low and relatively constant in both M forest types (x̄ = 0.5-2.0 kg/ha). In contrast, fruit production increased each year in R, and it was 5.0 to 19.6 times greater in R than in M stands beginning 3–5 years postharvest. Two disturbance-associated species, pokeweed (Phytolacca americana) and blackberry (Rubus allegheniensis), produced a large proportion of fruit in R but showed different patterns of establishment and decline. Huckleberry (Gaylussacia ursina) recovered rapidly after harvest and was a major producer in both silvicultural treatments and forest types each year. Several herbaceous species that are not associated with disturbance produced more fruit in CHR. Few species produced more fruit in M than in R. Fruit production by most tree species was similar between R and M, due to fruiting by stump sprouts in R within 1–3 years postharvest. Fruit availability was highest during summer and early fall. American holly (Ilex opaca), sumac (Rhus spp.), and greenbriar (Smilax spp.) retained fruit during winter months but were patchy in distribution. In the southern Appalachians, young recently regenerated stands provide abundant fruit compared to mature forest stands and represent an important source of food for wildlife for several years after harvest. Fruit availability differs temporally and spatially because of differences in species composition, fruiting phenology, and the dynamic process of colonization and recovery in recently harvested stands. Land managers could enhance fruit availability for many game and nongame species by creating or maintaining young stands within forests.     

Production, turnover and mycorrhizal colonization of root systems of three Populus species grown under elevated CO2 (POPFACE)

A fast growing high density Populus plantation located in central Italy was exposed to elevated carbon dioxide for a period of three years. An elevated CO₂ treatment (550 ppm), of 200 ppm over ambient (350 ppm) was provided using a FACE technique. Standing root biomass, fine root turnover and mycorrhizal colonization of the following Populus species was examined: Populus alba L., Populus nigra L., Populus x euramericana Dode (Guinier). Elevated CO₂ increased belowground allocation of biomass in all three species examined, standing root biomass increased by 47–76% as a result of FACE treatment. Similarly, fine root biomass present in the soil increased by 35–84%. The FACE treatment resulted in 55% faster fine root turnover in P. alba and a 27% increase in turnover of roots of P. nigra and P. x euramericana. P. alba and P. nigra invested more root biomass into deeper soil horizon under elevated CO₂. Response of the mycorrhizal community to elevated CO₂ was more varied, the rate of infection increased only in P. alba for both ectomycorrhizal (EM) and arbuscular mycorrhizas (AM). The roots of P. nigra showed greater infection only by AM and the colonization of the root system of P. x euramericana was not affected by FACE treatment. The results suggest that elevated atmospheric CO₂ conditions induce greater belowground biomass investment, which could lead to accumulation of assimilated C in the soil profile. This may have implications for C sequestration and must be taken into account when considering long‐term C storage in the soil.     

Stable fly, Stomoxys calcitrans, mouthpart removal influences stress and anticipatory responses in mice

Abstract Biting fly attack induces a variety of stress and anxiety related changes in the physiology and behaviour of the target animals. Significant reductions in pain, or more appropriately, nociceptive sensitivity (latency of a foot-lifting response to an aversive thermal stimulus), are evident in laboratory mice after a 1 h exposure to stable flies, Stomoxys calcitrans. The role of the various components of biting fly attack in the development of this stress-induced reduction in pain sensitivity (analgesia) is, however, unclear. This study demonstrates that fly-naive mice do not exhibit a stress-induced analgesia when exposed to stable flies whose biting mouthparts have been removed. In contrast, mice that have been previously exposed to intact stable flies exhibit significant analgesia when exposed to flies that are incapable of biting. However, the level of analgesia induced is lower than that elicited by exposure to intact stable flies. Exposure to non-biting house flies, Musca domestica , has no effect on nociceptive sensitivity. It appears that the actual bite of the stable fly is necessary for the induction of analgesia and probably other stress and anxiety associated responses in fly naive mice. However, mice rapidly learn to recognize biting flies and exhibit significant, possibly anticipatory analgesic responses to the mere presence of biting flies.